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Tucker Lab

Research News

• Disease modeling: Using iPSCs to model retinal disease and identify the role of a new disease causing gene in the pathophysiology of RP.

• Stem cell transplantation: Development of an iPSC based approached for the treatment of retinal degenerative disease.

• Tissue engineering: Using biodegradable polymer based grafts to remove retinal scaring, promote retinal stem cell integration, and induce retinal regeneration.

• Dr.Tucker and co-investigator John Fingert awarded a National Glaucoma Research grant of $100,000 for their research project: Stem Cell Approaches to Glaucoma from BrightFocus (Formerly American Health Assistance Foundation).

Program in Stem Cell Biology

Medical Education and  Biomedical Research Facility
Dr. Erin Burnight feeding cells in our stem cell culture facility.
Dr. Budd Tucker isolating induced pluripotent stem cells, from a retinal degerative patient.
Tucker Stem Cell Lab Research Team.
Medical Education and  Biomedical Research Facility

Tucker Laboratory Research

  • RPC Transplantation

  • Photoreceptor Sheets

  • Teratoma Characterization

  • Teratoma Formation

  • Retinal Gliosis

  • Adult Mouse iPSCs

  • iPSC Generation

  • Retinal Transplantation

RPC Transplantation

RPC Transplantation

Retinal progenitor cells break through the glial scar and integrate
within the host dystrophic retina post-transplantation

 

Photoreceptor Sheets

Photoreceptor Sheets

The intact laminar structure of a photoreceptor sheet makes it an
attractive tissue for ocular transplantation

Teratoma Characterization

Teratoma Characterization

Teratomas contain tissues of all 3 germ layers including vascular tubes

Teratoma Formation

Teratoma Formation

Formation of a large teratoma at 4 weeks post injection of
undifferentiated iPSCs into a mouse eye

Retinal Gliosis

Retinal Gliosis

Retinal degeneration stimulates muller cell activity and glial scar formation at the outer
limits of the diseased retina

Adult Mouse iPSCs

Adult Mouse iPSCs

iPSCs generated from adult mice expressing the red fluorescent reporter dsRed

iPSC Generation

iPSC Generation

Mitotically active fibroblast cell being reprogramed

Retinal Transplantation

Retinal Transplantation

Bipolar cells making connections with newly engrafted iPSCs